Sequence analysis fnr genes
were identified by BLASTP (http://www.blast.ncbi.nlm.nih.gov/Blast.cgi) Selleckchem AZD1480 homology searching in the genomes of MSR-1 (GenBank: CU459003.1), M. magneticum (GenBank AP007255.1), M. magnetotacticum (NCBI reference sequence NZ_AAAP00000000.1), Mc. marinus (GenBank accession number CP000471.1), and D. magneticus strain RS-1 (GenBank accession number AP010904.1). ClustalW was used for sequence alignment. The identification of Fnr S63845 order binding sites in the promoter regions of the different operons encoding denitrification enzymes were performed with the virtual footprint software (PRODORIC, http://www.prodoric.tu-bs.de/vfp/index2.php). Acknowledgements We thank Kirsten Jung, Ludwig-Maximilians-Universität München, for strain ΔEcfnr mutant. The China Scholarship Council (CSC) is greatly acknowledged for the financial support of Y. Li, and the Brazilian CNPq program for the financial support of K. T. Silva. This work was supported by grants DFG Schu1080/11-1 and 15–1, and HFSP RGP0052/2012
to D. Schüler. Electronic supplementary material Additional file 1: Magnetosome formation in WT overexpressing MgFnr. Plasmid pLYJ110 and pLYJ153 contains fnr gene from MSR-1 and E. coli, respectively. Cells were grown in anaerobic nitrate medium. Bar, 100 nm. (PDF 88 KB) Additional file 2: Detection of Fnr binding sites selleck in the upstream regions of nap , nirS , nor , and nosZ . The putative Fnr binding sites in the promoter regions are indicated
in yellow. (PDF 85 KB) Additional file 3: Transcription of nosZ fused to gusA in Mgfnr variant strains under microaerobic in the presence of nitrate. Expression was measured by β-glucuronidase activity. (PDF 179 KB) Additional file 4: Magnetosome formation in different Mgfnr variant strains. Cells were grown in microaerobic nitrate medium. Bar, 100 nm. Irregular shaped particles are indicated by black arrows. (PDF 265 KB) Additional file 5: Bacterial strains and plasmids used in this work. (PDF 190 KB) References 1. Jogler C, Schüler D: Genomics, genetics, and cell biology Tacrolimus (FK506) of magnetosome formation. Annu Rev Microbiol 2009, 63:501–521.PubMedCrossRef 2. Ullrich S, Kube M, Schübbe S, Reinhardt R, Schüler D: A hypervariable 130-kilobase genomic region of Magnetospirillum gryphiswaldense comprises a magnetosome island which undergoes frequent rearrangements during stationary growth. J Bacteriol 2005, 187:7176–7184.PubMedCentralPubMedCrossRef 3. Murat D, Quinlan A, Vali H, Komeili A: Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. Proc Natl Acad Sci U S A 2010, 107:5593–5598.PubMedCentralPubMedCrossRef 4. Lohsse A, Ullrich S, Katzmann E, Borg S, Wanner G, Richter M, Voigt B, Schweder T, Schüler D: Functional analysis of the magnetosome island in Magnetospirillum gryphiswaldense : the mamAB operon is sufficient for magnetite biomineralization.
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