These primers are lying in exon 11 and therefore detect both isoforms forms together. Sequence of M2-Pk (NM_011099) was fetched from Entrez Nucleotide database on NCBI http://www.ncbi.nlm.nih.gov. (PDF 12 KB) Additional file 4: Number of cells of hepatic sinusoids raised in CDE treated mice.
Cells of hepatic sinusoids were depicted by immunohistochemistry with an anti-F4/80 antibody (Kupffer cell, A, A’), an anti-vimentin-antibody (mesenchymal cells, B, B’), an anti-nestin antibody (activated HSCs, C, C’) and an anti-CD31 (marker of defenestrated endothelial cells, D, D’). Bar = 50 μm. (TIFF 9 MB) References 1. Shinozuka H, Lombardi B, Sell S, Iammarino RM: Early histological and functional EX 527 concentration alterations of ethionine liver carcinogenesis in rats fed a choline-deficient diet. Cancer Res 1978, 38:1092–1098.PubMed 2. Lim R, Knight B, Patel
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