They were observed using a scanning electron microscope (SEM) and treated via a critical point drying technique after glutaraldehyde (for fixation) and osmium tetroxide (for contrast enhancement) treatments. Results and discussion Si nanowires were chosen as building blocks to probe neural cells because crucial factors for intracellular interfacing, such
as their diameter, length, etc., can be easily tuned. Moreover, our previous study indicated that Si nanowires are bio-compatible to excitable cells (hippocampal neurons) and are thus safe for interfacing [26]. It is known that the cell process is critically affected by the surface that the cells come into contact with [28–30]. In our study, the nanowire population density, diameter, and length were investigated because they determine the surface structure of the substrate. Figure 1a,b,c shows nanowires MM-102 molecular weight grown on substrates with densities of Figure 1a 2.5 × 104 mm−2, Figure 1b 1.5 × 105 mm−2, and Figure 1c 1.5 × 106 mm−2. Figure 1d,e,f,g shows SEM images of GH3 cells cultured on bare silicon substrate and the
three substrates noted above for 72 h. In the bare silicon substrate, as shown in Figure 1d, GH3 cells were attached loosely to the silicon surface and grew close to other cells. Figure 1e,f,g shows that the cell body appeared to be widely stretched and attached {Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleck Anti-cancer Compound Library|Selleck Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Selleckchem Anti-cancer Compound Library|Selleckchem Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|Anti-cancer Compound Library|Anticancer Compound Library|buy Anti-cancer Compound Library|Anti-cancer Compound Library ic50|Anti-cancer Compound Library price|Anti-cancer Compound Library cost|Anti-cancer Compound Library solubility dmso|Anti-cancer Compound Library purchase|Anti-cancer Compound Library manufacturer|Anti-cancer Compound Library research buy|Anti-cancer Compound Library order|Anti-cancer Compound Library mouse|Anti-cancer Compound Library chemical structure|Anti-cancer Compound Library mw|Anti-cancer Compound Library molecular weight|Anti-cancer Compound Library datasheet|Anti-cancer Compound Library supplier|Anti-cancer Compound Library in vitro|Anti-cancer Compound Library cell line|Anti-cancer Compound Library concentration|Anti-cancer Compound Library nmr|Anti-cancer Compound Library in vivo|Anti-cancer Compound Library clinical trial|Anti-cancer Compound Library cell assay|Anti-cancer Compound Library screening|Anti-cancer Compound Library high throughput|buy Anticancer Compound Library|Anticancer Compound Library ic50|Anticancer Compound Library price|Anticancer Compound Library cost|Anticancer Compound Library solubility dmso|Anticancer Compound Library purchase|Anticancer Compound Library manufacturer|Anticancer Compound Library research buy|Anticancer Compound Library order|Anticancer Compound Library chemical structure|Anticancer Compound Library datasheet|Anticancer Compound Library supplier|Anticancer Compound Library in vitro|Anticancer Compound Library cell line|Anticancer Compound Library concentration|Anticancer Compound Library clinical trial|Anticancer Compound Library cell assay|Anticancer Compound Library screening|Anticancer Compound Library high throughput|Anti-cancer Compound high throughput screening| tightly as the population density of nanowires increases. selleckchem In the case of the substrate with the low population density of nanowires, most of the cells grew normally and displayed a morphology equivalent in quality to that grown on the
bare silicon substrate without regard to nanowire interfacing. In the case of the interfacing with the high population density of nanowires, we observed some cells with a holey membrane as shown in Figure 1g, indicating a loss of their functions. This means that GH3 cells failed to withstand wiring damage. Figure 1 Scanning electron microscope images of Si nanowires and GH3 cells. (a,b,c) Typical SEM images of Si nanowires grown on a Si substrate with various wire densities ((a) 2.5 × 104 mm−2, (b) 1.5 × 105 mm−2, (c) Rebamipide 1.5 × 106 mm−2). (d,e,f) SEM images of GH3 cells cultured on plane Si and nanowire-grown substrates shown in (a), (b), and (c). (g) SEM images of GH3 cells cultured on Si nanowire-grown substrates with high population density. To verify how nanowire interfacing affects the cell viability, an MTT assay, a technique widely used to measure cell viability, was performed under the same conditions. Additional file 1: Figure S2 shows that the activity of the GH3 cell interfaced with a certain nanowire density and culture time is higher than that cultured on the bare silicon substrate. It also shows that too many interfaces with nanowires can have an adverse effect on the cell viability. We investigated the effect of the population density of the nanowires on the growth of primary hippocampal neurons.
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