We then aligned the confocal and two-photon image stacks and determined whether the recorded compartments were axon terminals of bipolar cells, processes of starburst cells, or dendrites of ON DS cells (Figures 3A, 3B, and S3). Starburst processes could be identified because they were double-positive for GFP and ChAT. Strikingly, we found that none of the axon terminals of the 17 type-5 bipolar PLX3397 in vitro cells that belonged to the local circuit of nine ON DS
cells that were recorded were direction selective: they responded in a similar way to all directions (Figures 3C–3J). The lack of direction selectivity was not only observed in averaged signals (three repetitions) but also in individual responses and we did not find response failures (Figure S3). The response vector of bipolar terminals pointed in random directions, neither aligned with the preferred direction of the connected ON DS cell nor with any other cardinal directions (Figures 3H and 3I). Some type-5 bipolar terminals provide synaptic input to ON DS cells, while others may drive different ganglion cell types; however, we found no direction-selective activity even when the analysis was restricted to those bipolar terminals
that were positioned next to ON DS cell dendrites (Figures 4E–4G), suggesting selleck chemical that activity in all identified type-5 bipolar cell terminals are direction nonselective. To simultaneously observe the concerted activity of ON DS-connected bipolar terminals, starburst cell processes,
and ON DS dendrites during motion stimulation, we imaged retinal regions around an ON DS cell where all of these three elements were labeled. It was possible to visualize the synaptic compartments of the circuit simultaneously, since these compartments are restricted Glutathione peroxidase to one two-photon image plane in the inner plexiform layer of the retina (Figures 4A and 4B). The simultaneous imaging of subcellular compartments clearly showed the different behavior of the three circuit elements: the axon terminals of bipolar cells were not direction selective, the processes of starburst cells showed “local” direction selectivity along the centrifugal axis (Euler et al., 2002), and the dendrites of ON DS cells were all “globally” direction selective along the same axis (Figures 4C and 4D). Neurotransmission from bipolar cells to ganglion cells is mediated by glutamate. To directly test whether the glutamate input signal to ON DS cells is direction selective or not, we developed a G-deleted rabies virus expressing a glutamate sensor iGluSnFR (Borghuis et al., 2013 and Marvin et al., 2013) and injected it into the medial terminal nucleus (Figure 4H).
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