25 mm diameter and 0.25 μm film thickness. The column oven temperature was programmed from 50 °C to 300 °C for 2 °C min−1. Ionization of the sample components was performed in electron impact mode (EI, 70 eV). The temperature of the injector was fixed to 240 °C and one of the detectors to 200 °C. Helium (99.99% purity) was the carrier gas fixed with a flow rate of 1.51 mL min−1. The mass range from 40 to 1000 m/z was scanned at a rate of 3.0 scans/s. 1.0 μL of the methanol, chloroform and ethanol extracts of C. decandra was injected with a Hamilton syringe www.selleckchem.com/products/scr7.html to the GC–MS manually for total ion chromatographic analysis in split
injection technique. Total running time of GC–MS is 35 min. The relative percentage of the each extract constituents was expressed as percentage with peak area normalization. The spectrum of the unknown component was compared with
the spectrum of the known components stored in the NIST08s, WILEY8, and FAME libraries and was ascertained the name, molecular weight and structure of components of the test materials. The results obtained were interpreted. The mangrove plant C. decandra leaves were powdered using mechanical grinder and crude extracts were obtained by Soxhlet using chloroform, methanol Neratinib and ethanol. Specific concentrations of the crude compounds were obtained by dissolved in DMSO. The antifungal activity of crude extracts of C. decandra leaves was determined in vitro by Agar cup bioassay method against phytopathogenic fungi P. aphanidermatum, R. solani, P. oryzae and F. oxysporum by calculating the zone of Inhibition around the well. Among all leaf extracts, chloroform extracts of C. decandra leaves showed strong antifungal against P. aphanidermatum, R. solani, P. oryzae, C. oryzae and F. oxysporum
with zone of inhibition diameter (IZD) of 29 mm, 27 mm, 28 mm, found 28 mm and 28 mm, respectively at a concentration of 500 μg/mL. 25 mm, 24 mm, 22 mm, 25 mm and 23 mm of zone of inhibition diameter (IZD) showed respectively against P. aphanidermatum, R. solani, P. oryzae, C. oryzae and F. oxysporum at a concentration of 250 μg/mL. Methanolic extracts also showed highest antifungal activity next to chloroform extracts against P. aphanidermatum, R. solani, P. oryzae, C. oryzae and F. oxysporum with zone of inhibition diameter (IZD) of 27 mm, 28 mm, 25 mm, 26 mm and 27 mm respectively at 500 μg/mL concentration and 21 mm, 22 mm, 17 mm, 20 mm, 20 mm respectively at 250 μg/mL concentration. Ethanol extracts exhibited moderate activity showed against P. aphanidermatum, R. solani, P. oryzae, C. oryzae and F. oxysporum with zone of inhibition diameter (IZD) of 20 mm, 22 mm, 22 mm, 24 mm and 23 mm respectively at 500 μg/mL concentration. Clotrimazole exhibited higher degree of antifungal activity at a concentration of 50 μg/mL, when compared to higher concentrations of the test compounds. The antifungal activity of organic solvent extracts of C.
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