In previous study, the concentration of adenovirus receptor in the liver was high, so as the distribution of adenovirus vector[18]. Some studies on the homing behavior of hemopoietic stem cells showed that part of the transplanted cells stayed in spleen for a time, [19] while others reported the number of donor cells in spleen kept at a low level at all times in nonablated mice[20]. In our study, human MDR1 and P-gp were not detected in liver and spleen of any group. Maybe there were not enough niches in our study. In further research human MDR1 would be detected by taking shorter time, such as 12 hours or 1 day after transplantation and be analyzed through
more sensitive methods. this website Some study reported that systemically administered adenovirus vector had been shown inhibition of myeloid progenitor growth, inducing transient leucopenia and thrombocytopenia[21]. In this study, our data of blood cell counts did not support a role for MDR1-BMCs in dysregulated haemopoiesis in short term posttranplantation. It had been reported that adenovirus vectors eliciting the humoral immune response for many years[22]. And many factors would influence immune responses, like route of administration, dose of vector, host and so on. In this study, Day 7 after BMT was chosen
Go6983 cell line to investigate the humoral response after administration, because some researchers reported that SNF increased and reached peak levels at Day 7 after local administration[11]. Our results showed that no SNF was detected after transplantation and the levels of adenovirus-specific antibody also had no significance among each group. It indicated that the adenovirus vector did not have notable effect on immune response.
In some other studies, adenovirus vector were administered by intravenous injection, Tobramycin intra-arterial injection or localized delivery routes[23]. Adenovirus was detected in the injection site or major organs[24], proinflammatory cytokine was also detected in the serum, and inflammatory response appeared at and near the site of injection. Their data showed that SNF and anti-adenovirus antibody levels had been elevated postadministration[25, 26]. We considered that these differences were caused by the differences of delivery routes. Studies with IBM-BMT showed it induced persistent donor specific tolerance in mice even if the radiation doses were reduced to sublethal levels. And it was good for allogeneic BMT, because no GVHD developed, no graft failure occurred when the radiation dose was low, and hemopoietic recovery was rapid[27]. Conclusions Enhanced BMCs clearance of Sirolimus purchase pharmaceuticals via P-gp may reduce plasma concentrations and in turn the therapeutic efficacy of these agents. It remained technically feasible that drug resistance gene was able to protect haemopoiesis from the side effects of cytotoxin in chemotherapy[28].
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