This non-destructive measurement method provides localization of adducts within cells in reasonable time and cost and multiple samples can be processed in a batch employing defined conditions. Absence of BPDE-DNA adducts were observed in tissue sections from liver and lungs of mice receiving vehicle or dietary curcumin whereas significant as well as measurable levels of BPDE-DNA adducts were observed in these tissues following 24 h of B(a)P administration as reported in mouse skin, liver and lungs [7], [20] and [21]. The
time-dependent [BP(+48h), BP(+96h), BP(+144h)/BP (+8d), BP(+15d), BP(+29d)] decrease in the levels of BPDE-DNA adducts in the liver and lungs PS-341 selleck compound compared to BP(+24h) following the single dose of carcinogen
exposure was similar to that observed by others investigators in mouse/rat skin during 24 h–28 days [20] and [22]. The time-related decrease in the levels of DNA adducts was relatively higher in the liver than the lungs compared to BP(+24h). Our results clearly demonstrate that dietary curcumin (0.05%) post-treatment [subgroups BP(+48h) + C 24 h, BP(+96h) + C 72 h, BP(+144h) + C 120 h and BP(+8d) + C 7d, BP(+15d) + C 14d, BP(+29d) + C 28d in experiments 1 and 2, respectively] further enhanced the decrease in the levels
of BPDE-DNA adducts both in the liver and lungs at 48-144 h (experiment 1) and 8-28 days (experiment 2) compared to BP(+24h) and respective time-matched controls [subgroups BP(+48h), BP(+96h), BP(+144h) and BP(+8d), BP(+15d), BP(+29d) in experiments 1 and 2, respectively]. In the present study the observation of high levels of BPDE-DNA adducts at 24 h after the carcinogen treatment and sharp decreases within 1 wk (∼8 days) is also in agreement with observations Fossariinae reported on mouse/rat skin [22]. The probable reasons for the observed time-related decrease in the levels of BPDE-DNA adducts in the liver and lungs could be due to loss or turnover of DNA adduct containing cells and/or repair of carcinogen-DNA adducts and/or dilution of adducted DNA with newly synthesized non-adducted DNA. The observed curcumin-mediated enhancement in the disappearance of BPDE-DNA adducts is likely to be due to modulation of one or more of the aforementioned processes. Analyses conducted to identify the reasons for curcumin-mediated enhanced disappearance of BPDE-DNA adducts showed that basal levels of apoptosis/turnover in the control liver (5-10%) and lungs (20-35%) were significantly enhanced by a single dose of B(a)P only in the liver (17-24%) but not in the lungs (32-38%).
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